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Role of natural killer cells in the progression of Mycobacterium tuberculosis infection to pulmonary disease

Abreu, Mónica Teresa Parente
Fonte: Universidade de Coimbra Publicador: Universidade de Coimbra
Tipo: Dissertação de Mestrado
POR
Relevância na Pesquisa
925.3673%
Introduction: Natural killer (NK) cells are key components of the innate immune system and participate in the early response against infected or transformed cells. They are characterized by the expression of a varied repertoire of receptors, named inhibitors and activators, which balance mediates their function. NK cell contribution in infection remains unclear. Mycobacterium tuberculosis infection is still a significant health problem and it will probably become even more significant in coming years because of the high prevalence of human immunodeficiency virus (HIV). The main goal is to clarify the role of NK cells in immunopathogenesis and hypothetical contribution as targets for therapy interventions. Material and methods: Peripheral blood from 38 TB patients and 15 HC was analysed. Complete Blood Cells count as well as enumeration of lymphocyte subsets was made. NK cell surface expression of some important receptors and markers (CD56/CD16, CD27/CD11b, CD57, CD94/NKG2D, NKp30, NKp44, NKp46, NKp80, KIR2DL1, KIR2DL2, KIR3DL1, KIR2DS1) were evaluated and also the intracellular expression of IFN-γ. Results and Discussion: A moderate lymphopenia was observed in TB patients, with a decrease of all lymphocyte subsets, except for B cells. NK cells from TB patients present a higher level of maturation and IFN-γ production. The most marked increase in surface markers expression was observed for CD57. Conclusion: The general NK cells phenotyping here presented can help in the understanding of NK cell role in pulmonary tuberculosis infection and progression to disease...

Leukotrienes are not essential for the efficacy of a heterologous vaccine against Mycobacterium tuberculosis infection

FRANCO, L.H.; PAULA, M. Oliveira e; WOWK, P.F.; FONSECA, D.M. da; SÉRGIO, C.A.; FEDATTO, P.F.; GEMBRE, A.F.; RAMOS, S.G.; SILVA, C.L.; MEDEIROS, A.I.; FACCIOLI, L.H.; BONATO, V.L.D.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
819.0007%
Leukotrienes are reported to be potent proinflammatory mediators that play a role in the development of several inflammatory diseases such as asthma, rheumatoid arthritis and periodontal disease. Leukotrienes have also been associated with protection against infectious diseases. However, the role of leukotrienes in Mycobacterium tuberculosis infection is not understood. To answer this question, we studied the role of leukotrienes in the protective immune response conferred by prime-boost heterologous immunization against tuberculosis. We immunized BALB/c mice (4-11/group) with subcutaneous BCG vaccine (1 x 10(5) M. bovis BCG) (prime) followed by intramuscular DNA-HSP65 vaccine (100 µg) (boost). During the 30 days following the challenge, the animals were treated by gavage daily with MK-886 (5 mg·kg-1·day-1) to inhibit leukotriene synthesis. We showed that MK-886-treated mice were more susceptible to M. tuberculosis infection by counting the number of M. tuberculosis colony-forming units in lungs. The histopathological analysis showed an impaired influx of leukocytes to the lungs of MK-886-treated mice after infection, confirming the involvement of leukotrienes in the protective immune response against experimental tuberculosis. However...

TLR2-dependent mast cell activation contributes to the control of Mycobacterium tuberculosis infection

CARLOS, Daniela; FRANTZ, Fabiani G.; SOUZA-JUNIOR, Devandir A.; JAMUR, Maria C.; OLIVER, Constance; RAMOS, Simone G.; QUESNIAUX, Valerie F.; RYFFEL, Bernhard; SILVA, Celio L.; BOZZA, Marcelo T.; FACCIOLI, Lucia H.
Fonte: ELSEVIER SCIENCE BV Publicador: ELSEVIER SCIENCE BV
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
814.53984%
Mast Cells (MCs) express toll-like receptor 2 (TLR2), a receptor known to be triggered by several major mycobacterial ligands and involved in resistance against Mycobacterium tuberculosis (MTB) infection. This study investigated whether adoptive transfer of TLR2 positive MCs (TLR2(+/+)) corrects the increased susceptibility of TLR2(-/-) mice to MTB infection. TLR2(-/-) mice displayed increased mycobacterial burden, diminished myeloid cell recruitment and proinflammatory cytokine production accompanied by defective granuloma formation. The reconstitution of these mice with TLR2(+/+) MCs, but not TLR2(-/-), confers better control of the infection, promotes the normalization of myeloid cell recruitment associated with reestablishment of the granuloma formation. In addition, adoptive transfer of TLR2(+/+) MC to TLR2(-/-) mice resulted in regulation of the pulmonary levels of IL-beta, IL-6, TNF-alpha, enhanced Th1 response and activated CD8(+) T cell homing to the lungs. Our results suggest that activation of MCs via TLR2 is required to compensate the defect in protective immunity and inability of TLR2(-/-) mice to control MTB infection. (C) 2009 Elsevier Masson SAS. All rights reserved.; FAPESP[03/12885-5]

Reactive nitrogen intermediate susceptibility of Mycobacterium tuberculosis genotypes in an urban setting

INUMARU, V. T. G.; NOGUEIRA, P. A.; BUTUEM, I. V.; RILEY, L. W.; FERRAZOLI, L.
Fonte: INT UNION AGAINST TUBERCULOSIS LUNG DISEASE (I U A T L D) Publicador: INT UNION AGAINST TUBERCULOSIS LUNG DISEASE (I U A T L D)
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
815.2934%
BACKGROUND: Mycobacterium tuberculosis genotypes resistant to reactive nitrogen intermediates (RNI) predominate in certain urban communities, suggesting that this phenotype influences disease transmission. OBJECTIVE: To compare different M. tuberculosis genotypes for resistance to RNI generated in vitro. DESIGN: We genotyped 420 M. tuberculosis isolates from a neighborhood in Sao Paulo, Brazil, and analyzed them for susceptibility to RNI generated in acidified sodium nitrite (ASN) solution. RESULTS: Seventy-one (43%) of 167 recent-infection strains and 68 (43%) of 158 endogenous infection strains showed moderate- to high-level ASN resistance. CONCLUSION: ASN resistance of M. tuberculosis is not necessarily a determining factor for enhanced transmission.; Conselho Nacional cle Desenvolvimento Cientifico e Tecnologico (CNPq); NIH Fogarty International Center[TW006563]

Estudo dos mecanismos de virulência de cepas resistentes e sensíveis de Mycobacterium tuberculosis; Study of mechanisms of virulence of resistant and susceptible strains of Mycobacterium tuberculosis

Couto, Jamile
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 14/11/2008 PT
Relevância na Pesquisa
731.7576%
A tuberculose continua sendo um grave problema de saúde pública mundial. O principal agente etiológico da doença é o Mycobacterium tuberculosis, infectando entre outras células, os macrófagos. A infecção pode manter-se latente por vários anos sem causar sintomas clínicos aparentes. Para esta pesquisa, foi proposto o estudo de indução de marcadores de resposta inflamatória em células THP-1 diferenciadas em macrófagos utilizando isolados micobacterianos sensíveis, uni ou multidrogas resistentes transfectados com o plasmídeo pFPCAGFP carreando o gene da proteína verde fluorescente, como marcador de infecção celular. Objetivou também avaliar a relação entre isolados clínicos de M.tuberculosis sensíveis ou com resistência a uma ou multidrogas e sua relação à virulência. Para isso, culturas da linhagem monocítica humana (THP-1) foram mantidas para posterior infecção por isolados clínicos, genotipicamente identificados pertencentes à nossa micobacterioteca. A expressão do RNAm foi quantificada e avaliada por RTPCR e posteriormente, PCR em tempo real foi realizado. Após infecção das células THP-1 diferenciadas em macrófagos, observou-se que houve um aumento na expressão das citocinas pró-inflamatórias (IL-1β...

Determinação da sensibilidade e especificidade de teste de liberação de interferon-gama por linfócitos ativos estimulados por antígenos específicos do Mycobacterium tuberculosis em crianças; Evaluation of the sensibility and the specificity of an interferon-gamma release assay after lymphocyte stimulation by specific Mycobacterium tuberculosis antigens in children

Vallada, Marcelo Genofre
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 01/09/2009 PT
Relevância na Pesquisa
832.4646%
INTRODUÇÃO: A tuberculose é um problema grave de saúde pública, acometendo indivíduos em todas as faixas etárias e em todos os estratos socioeconômicos. Apesar de estarem sob grande risco de adoecimento, as crianças carecem de meios diagnósticos sensíveis e específicos. Neste estudo avaliou-se em crianças a acurácia de um teste baseado na dosagem de interferon-gama liberado por linfócitos após estímulo com antígenos específicos do Mycobacterium tuberculosis (QuantiFERON-TB Gold In Tube® [Cellestis, Carnegie, Austrália] ).MÉTODO: Foram incluídas no estudo 184 crianças não infectadas e 11 crianças com infecção pelo Mycobacterium tuberculosis. Todas as crianças receberam previamente o BCG. Foram excluídas crianças com comprometimento do sistema imunológico. Obteve-se amostra de sangue de cada criança, e o material foi processado conforme as instruções do laboratório fabricante. O desempenho do teste foi avaliado pela construção de uma curva de características operacionais (ROC). RESULTADOS: Do total de 184 crianças sem infecção pela micobatéria, 74 (40,2%) eram do sexo feminino, e 130 (70,6%) tinham menos de quatro anos de idade. A idade média neste grupo foi de 35 meses. Seis (3,2%) crianças apresentaram resultado indeterminado do teste...

Papel da proteína HspX do Mycobacterium tuberculosis na regulação de genes relacionados à adaptação morfológica de micobactérias ao período de dormência, utilizando Mycobacterium smegmatis como organismo modelo; The role of HspX protein from Mycobacterium tuberculosis in regulation of genes involved with morphological adaptation to mycobacterial dormancy, with Mycobacterium smegmatis as model organism.

Bastos, Gisele Medeiros
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 22/03/2013 PT
Relevância na Pesquisa
826.007%
A manutenção da infecção latente pelo M. tuberculosis (TBIL) pode ser atribuída à sua capacidade de sobreviver durante anos no organismo humano em um estado não replicativo (dormente). A proteína HspX do M. tuberculosis, induzida sob condições de hipóxia, está fortemente associada com a manutenção da viabilidade do bacilo na TBIL. O presente estudo tem como objetivo, verificar se a superexpressão da proteína HspX altera a expressão de genes envolvidos com a síntese de componentes da parede celular, replicação do DNA e divisão celular de bacilos, assim como, na expressão de genes envolvidos com a resposta imune inata em macrófagos infectados com esses bacilos. O gene hspX foi amplificado pela PCR a partir do DNA do M. tuberculosis H37Rv, clonado no vetor de expressão pFPCA1GFP, e a proteína HspX expressa em M. smegmatis mc2155. As bactérias, nas quais, a presença da proteína recombinante foi confirmada por Western Blot, foram utilizadas, para a análise de expressão gênica tanto em bactérias quanto em macrófagos infectados. O estudo de expressão gênica foi realizado utilizando a RT-qPCR. Quando comparado aos controles, as bactérias que expressavam a proteína HspX apresentaram uma redução na expressão de genes de replicação do DNA e divisão celular...

Avaliação da participação de mediadores lipídicos nas infecções experimentais induzidas por diferentes isolados de Mycobacterium tuberculosis de humanos; Evaluation of lipid mediators participation in the experimental infections induced by different isolates of Mycobacterium tuberculosis from human.

Soares, Elyara Maria
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 13/09/2013 PT
Relevância na Pesquisa
732.2825%
Os mecanismos que conferem resistência do Mycobacterium tuberculosis (Mtb) à destruição pelo hospedeiro, além da sua capacidade em permanecer e/ou multiplicar-se no interior das células fagocitárias são ainda pouco compreendidos. Nosso grupo de pesquisa tem contribuído para o entendimento do papel dos mediadores lipídicos, que incluem prostaglandinas (PGs) e leucotrienos (LTs) na tuberculose. PGs inibem a resposta imune celular TH1, a produção de citocinas e a fagocitose, e assim facilita a infecção. LTs estão envolvidos no recrutamento de leucócitos, e na modulação da síntese de citocinas, no aumento da fagocitose e dos mecanismos microbicidas, e assim contribui para a eliminação da micobactéria. Neste projeto, avaliamos in vivo e in vitro a produção dos mediadores lipídicos induzidos por cepas de Mtb isolados de pacientes com tuberculose ativa. Demonstramos neste trabalho que macrófagos alveolares infectados com os bacilos da cepa SV009 levam a maior produção de TNF- e nitrito, do que aqueles infectados com a cepa SV068. Em contraste, macrófagos alveolares infectados com os bacilos da cepa SV068 induzem a produção de muito mais LTB4, quando comparado aos bacilos da cepa SV009. Obtivemos maior recuperação de unidades formadoras de colônia (UFC) de macrófagos alveolares tratados com MK886 e infectados com bacilos da cepa SV068; enquanto que mais UFCs foram recuperadas após o tratamento com ácido caféico e infecção com a cepa SV009. Com relação a formação de corpúsculos lipídicos (CLs)...

Leukotrienes are not essential for the efficacy of a heterologous vaccine against Mycobacterium tuberculosis infection

Franco, L. H.; Paula, M. Oliveira e; Wowk, P. F.; Fonseca, D. M. da; Sérgio, C. A.; Fedatto, P. F.; Gembre, A. F.; Ramos, S. G.; Silva, C. L.; Medeiros, Alexandra Ivo de; Faccioli, L. H.; Bonato, V. L. D.
Fonte: Associação Brasileira de Divulgação Científica (ABRADIC) Publicador: Associação Brasileira de Divulgação Científica (ABRADIC)
Tipo: Artigo de Revista Científica Formato: 645-650
ENG
Relevância na Pesquisa
819.0007%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Processo FAPESP: 07/02407-0; Leukotrienes are reported to be potent proinflammatory mediators that play a role in the development of several inflammatory diseases such as asthma, rheumatoid arthritis and periodontal disease. Leukotrienes have also been associated with protection against infectious diseases. However, the role of leukotrienes in Mycobacterium tuberculosis infection is not understood. To answer this question, we studied the role of leukotrienes in the protective immune response conferred by prime-boost heterologous immunization against tuberculosis. We immunized BALB/c mice (4-11/group) with subcutaneous BCG vaccine (1 x 10(5) M. bovis BCG) (prime) followed by intramuscular DNA-HSP65 vaccine (100 µg) (boost). During the 30 days following the challenge, the animals were treated by gavage daily with MK-886 (5 mg·kg-1·day-1) to inhibit leukotriene synthesis. We showed that MK-886-treated mice were more susceptible to M. tuberculosis infection by counting the number of M. tuberculosis colony-forming units in lungs. The histopathological analysis showed an impaired influx of leukocytes to the lungs of MK-886-treated mice after infection...

Case report: Disseminated Mycobacterium tuberculosis infection in a dog

Martinho, Anna Paula Vitirito; Franco, Marília Masello Junqueira; Ribeiro, Márcio Garcia; Perrotti, Isabella Belletti Mutt; Mangia, Simone Henriques; Megid, Jane; Vulcano, Luiz Carlos; Lara, Gustavo Henrique Batista; Santos, Adolfo Carlos Barreto; Leite
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 596-600
ENG
Relevância na Pesquisa
917.4728%
An uncommon disseminated Mycobacterium tuberculosis infection is described in a 12-year-old female dog presenting with fever, dyspnea, cough, weight loss, lymphadenopathy, melena, epistaxis, and emesis. The dog had a history of close contact with its owner, who died of pulmonary tuberculosis. Radiographic examination revealed diffuse radio-opaque images in both lung lobes, diffuse visible masses in abdominal organs, and hilar and mesenteric lymphadenopathy. Bronchial washing samples and feces were negative for acid-fast organisms. Polymerase chain reaction (PCR)-based species identification of bronchial washing samples, feces, and urine revealed M. tuberculosis using PCR-restriction enzyme pattern analysis-PRA. Because of public health concerns, which were worsened by the physical condition of the dog, euthanasia of the animal was recommended. Rough and tough colonies suggestive of M. tuberculosis were observed after microbiological culture of lung, liver, spleen, heart, and lymph node fragments in Löwenstein-Jensen and Stonebrink media. The PRA analysis enabled diagnosis of M. tuberculosis strains isolated from organs. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.

Determinação dos genótipos para o polimorfismo dos receptores de IgG, FcγRlla (H/R131) e FcγRIIIb (NA1/NA2), e estudo do burst oxidativo dos neutrófilos em indivíduos infectados pelo HIV e/ou Mycobacterium tuberculosis

Faria, Carolina Maria Quinello Gomes de
Fonte: Universidade Estadual Paulista (UNESP) Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Dissertação de Mestrado Formato: 131 f. : il. + Apendice
POR
Relevância na Pesquisa
733.00195%
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR; A infecção pelo vírus da imunodeficiência humana (HIV) ocorre com aumento dos níveis de anticorpos séricos e com grande quantidade de imunocomplexos circulantes. A associação entre as disfunções da fagocitose mediadas por receptores do tipo FcR, na infecção pelo HIV e, principalmente, na co-infecção com o Mycobacterium tuberculosis, têm sido amplamente descrita na literatura científica. A tuberculose (TB) é a infecção oportunista mais comum entre pacientes infectados pelo HIV e a causa de morte mais frequente entre os pacientes com a síndrome da imunodeficiência adquirida (AIDS). Sabe-se que a infecção pelo HIV torna o paciente susceptível às infecções oportunistas, como a tuberculose, e, essas co-infecções aumentam o potencial de replicação viral. A ineficiência do mecanismo de clearance de imunocomplexo (IC) e de fagocitose pode contribuir para o aumento da suscetibilidade às infecções oportunistas em indivíduos infectados pelo HIV. Sendo assim, a determinação dos genótipos para as variantes polimórficas funcionais dos receptores para a porção Fc da imunoglobulina de classe G (FcR)...

Prova tuberculínica e QuantiFERON-TB Gold in-Tube na identificação da infecção latente pelo Mycobacterium tuberculosis em pessoas vivendo com AIDS; Tuberculin skin testing and QuantiFERON-TB Gold in-Tube in the identification of latent Mycobacterium tuberculosis infection in people living with AIDS; Prueba tuberculínica y Quantiferon-TB Gold in-Tube en la identificación de la infección latente por Mycobacterium tuberculosis en personas que viven con el sida

Souza, Josiane Maria Oliveira de
Fonte: Universidade de Brasília Publicador: Universidade de Brasília
Tipo: Tese
POR
Relevância na Pesquisa
735.6154%
Tese (doutorado)—Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Enfermagem, Programa de Pós-Graduação em Enfermagem, 2014.; A tuberculose (TB) é a mais freqüente e fatal doença oportunista em pessoas vivendo com HIV/aids (PVHA), em função do maior risco de seu desenvolvimento após a infecção pelo Mycobacterium tuberculosis. O diagnóstico e tratamento da infecção latente pelo Mycobacterium tuberculosis (ILTB) na PVHA é fundamental para evitar a progressão para doença e morte por TB. O presente estudo tem por objetivo analisar o uso dos testes Prova Tuberculínica (PT) e QuantiFERON-TB Gold in-Tube (QTF-GIT) na identificação da ILTB em pessoas vivendo com aids. A pesquisa utilizou os modelos epidemiológicos do tipo transversal, série de casos e estudo de caso. A amostra consistiu de 300 pacientes com HIV/aids, selecionados em oito serviços de assistência especializados em DST/HIV/aids do Distrito Federal, entre 2011 e 2013, com seguimento até maio de 2014. A análise dos dados transversais revelou uma média de CD4 de 477,5células/mm³, sendo que 18 pacientes (6%, IC95%:3,6-9,3) apresentaram ILTB a partir do resultado positivo da PT e/ou QTF-GIT. Destaca-se que quatro pacientes (1...

Leukotrienes are not essential for the efficacy of a heterologous vaccine against Mycobacterium tuberculosis infection

Franco,L.H.; Paula,M. Oliveira e; Wowk,P.F.; Fonseca,D.M. da; Sérgio,C.A.; Fedatto,P.F.; Gembre,A.F.; Ramos,S.G.; Silva,C.L.; Medeiros,A.I.; Faccioli,L.H.; Bonato,V.L.D.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/07/2010 EN
Relevância na Pesquisa
819.0007%
Leukotrienes are reported to be potent proinflammatory mediators that play a role in the development of several inflammatory diseases such as asthma, rheumatoid arthritis and periodontal disease. Leukotrienes have also been associated with protection against infectious diseases. However, the role of leukotrienes in Mycobacterium tuberculosis infection is not understood. To answer this question, we studied the role of leukotrienes in the protective immune response conferred by prime-boost heterologous immunization against tuberculosis. We immunized BALB/c mice (4-11/group) with subcutaneous BCG vaccine (1 x 10(5) M. bovis BCG) (prime) followed by intramuscular DNA-HSP65 vaccine (100 µg) (boost). During the 30 days following the challenge, the animals were treated by gavage daily with MK-886 (5 mg·kg-1·day-1) to inhibit leukotriene synthesis. We showed that MK-886-treated mice were more susceptible to M. tuberculosis infection by counting the number of M. tuberculosis colony-forming units in lungs. The histopathological analysis showed an impaired influx of leukocytes to the lungs of MK-886-treated mice after infection, confirming the involvement of leukotrienes in the protective immune response against experimental tuberculosis. However...

Fingerprinting breath: electrochemical monitoring of markers indicative of bacteria Mycobacterium tuberculosis infection

Metters,Jonathan P.; Kampouris,Dimitrios K.; Banks,Craig E.
Fonte: Sociedade Brasileira de Química Publicador: Sociedade Brasileira de Química
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2014 EN
Relevância na Pesquisa
812.7619%
Recently it has been shown that chemical markers in exhaled air/breath can provide a methodology for the detection of tuberculosis infection. These markers consist of methyl phenylacetate, methyl p-anisate, methyl nicotinate and o-phenylanisole (2-methoxybiphenyl). Current approaches utilise gas chromatography-mass spectrometry (GCMS) which are useful for centralised testing of breath samples. The World Health Organization (WHO) require a portable, non-invasive diagnostic tool for the screening of tuberculosis infection. In order to meet this, we demonstrate proof-of-concept for the analytical sensing of the identified chemical markers in aqueous solutions using electrochemical based technology. We demonstrate that screen-printed electrochemical sensors can be used as the basis of a diagnostic tool for the electrochemical breathprinting of chemical markers (methyl nicotinate and 2-methoxybiphenyl) useful for the screening of tuberculosis infection. It is hoped that further development will facilitate the potential for a portable, hand-held, non-invasive breath diagnostic tool to be realised.

Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals

Chetty, Shivan; Govender, Pamla; Pillay, Mona; Jaggernath, Manjeetha; Ndung’u, Thumbi; Klenerman, Paul; Mitchell, Jessica E.; Kasmar, Anne G.; Walker, Bruce David; Kasprowicz, Victoria Olivia
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
EN_US
Relevância na Pesquisa
800.9735%
Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 and CFP-10-specific IFN-(gamma) Elispot assay (RD1-Elispot) on 163 HIV-infected individuals living in a TB-endemic setting. An RD1-Elispot was performed every 3 months for a period of 3–21 months. 62% of RD1-Elispot negative individuals were positive by cultured Elispot. Fluctuations in T cell response were observed with rates of change ranging from −150 to +153 spot-forming cells (SFC)/200,000 PBMC in a 3-month period. To validate these responses we used an RD1-specific real time quantitative PCR assay for monokine-induced by IFN-(gamma) (MIG) and IFN-(gamma) inducible protein-10 (IP10) (MIG: r = 0.6527, p = 0.0114; IP-10: r = 0.6967, p = 0.0056; IP-10+MIG: r = 0.7055, p = 0.0048). During follow-up 30 individuals were placed on ARVs and 4 progressed to active TB. Fluctuations in SFC did not correlate with CD4 count, viral load, treatment initiation, or progression to active TB. The RD1-Elispot appears to have limited value in this setting.

INFECÇÃO PELO Mycobacterium tuberculosis ENTRE OS PROFISSIONAIS DA EQUIPE DE ENFERMAGEM, EM UM HOSPITAL DE DOENÇAS INFECCIOSAS, GOIÂNIA - GO.; Mycobacterium tuberculosis INFECTION AMONG PROFESSIONALS NURSING TEAM, AT A HOSPITAL FOR INFECTIOUS DISEASES, Goiânia - GO.

LOPES, Lilian Kelly de Oliveira
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Enfermagem; Cuidado em Enfermagem Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Enfermagem; Cuidado em Enfermagem
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
933.1899%
According to the World Health Organization (WHO), an hundred million of individuals are infected by M. tuberculosis, annually. Health care workers play an important role to control of tuberculosis, but they are also at high risk for this infection. Then, the objectives of the present study were to evaluate the prevalence of M tuberculosis infection in nursing professionals from the Tropical Diseases Hospital in Goiânia City, State of Goiás, to analyze the factors associated to tuberculin skin test (TST) positivity and to determine the TB infection incidence density in susceptible professionals Initially, the prevalence and factors associated to TST were investigated in 128 eligible individuals. Further, susceptible professionals (n=32) were followed up during three years (2001-2004) to detect TST conversion. Of the total individuals investigated, 69.5% (IC 95%: 60.7-77.2) were positive to TST. Two occupational factors were independently associated to skin test positivity: duration of profissional activity longer than 5 years (Adjustd OR = 6.3; 95% CI: 1.5-26.2) and occupational contact with a person with pulmonary TB ≤ 2 years (Adjusted OR = 12.2; 95% CI: 1.2-106.3). Seven profissionals showed tuberculinic conversion during the three years of follow up...

Mycobacterium smegmatis recombinante expressando a proteína CMX induz resposta imune contra Mycobacterium tuberculosis em camundongos BALB/c; Recombinant Mycobacterium smegmatis expressing CMX pretein induces immune response against Mycobacterium tuberculosis in BALB/c mice

Oliveira, Fábio Muniz de
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP); Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP); Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
734.1074%
For hundreds years tuberculosis (TB), a contagious disease caused by Mycobacterium tuberculosis (Mtb), has been a global public health problem. Even after the development of the vaccine BCG, in 1921, tuberculosis control continues on slow pace. This comes to be as a result of the variable efficacy (from 0 to 80%) presented by the vaccine in the protection against TB in adults. Therefore, the development of a new vaccine against TB is necessary. In this study, it was evaluated a recombinant vaccine composed of Mycobacterium smegmatis expressing the CMX fusion protein (mc2- CMX), formed from three antigen epitopes of Mtb: Ag85C, MPT51 and HspX. M. smegmatis mc2 155 was transformed with pLA71-CMX by electroporation, and the presence of the CMX protein was confirmed by imuno blotting. BALB/c mice were distributed in four groups: saline, infection, BCG and mc2-CMX. The groups were immunized with their respective vaccines in two moments with an interval of fifteen days and the animal blood was collected fifteen days after the last immunization. Thirty days after the last immunization, the animals were challenged with Mtb H37Rv (intravenously) and thirty days after the challenge, the blood was collected to perform ELISA test. Seventy days after the challenge...

Prevalence and evolution of Mycobacterium tuberculosis infection in tuberculosis case contacts

Albanese,Silvia Paulino Ribeiro; Costa,Arlete Alves Nunes Fragoso da; Pieri,Flávia Meneguetti; Alves,Elaine; Santos,Danielle Talita dos; Kerbauy,Gilselena; Arcêncio,Ricardo Alexandre; Dessunti,Elma Mathias
Fonte: Sociedade Brasileira de Medicina Tropical - SBMT Publicador: Sociedade Brasileira de Medicina Tropical - SBMT
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2015 EN
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814.1968%
INTRODUCTION : The tuberculin test is a diagnostic method for detecting latent tuberculosis (TB) infection, especially among disease contact cases. The objective of this study was to analyze the prevalence and evolution of Mycobacterium tuberculosis infection among TB contact cases. METHODS : A retrospective cohort study was performed in a reference center for TB. The study population consisted of 2,425 patients who underwent a tuberculin test from 2003 to 2010 and whose results indicated contact with individuals with TB. The data were collected from the registry book of the tuberculin tests, patient files and the Information System Records of Notification Grievance. To verify the evolution of TB, case records through September 2014 were consulted. Data were analyzed using the Statistical Package for the Social Sciences (SPSS). In all hypothesis tests, a significance level of 0.05 was used. RESULTS : From the studied sample, 435 (17.9%) contacts did not return for reading. Among the 1,990 contacts that completed the test, the prevalence of latent TB infection was 35.4%. Of these positive cases, 50.6% were referred to treatment; the dropout rate was 42.5%. Among all of the contacts, the TB prevalence was 1.8%, from which 13.2% abandoned treatment. CONCLUSIONS : The collected data indicate the need for more effective public policies to improve TB control...

Prevalence and incidence of Mycobacterium tuberculosis infection in nursing students in Vitória, Espírito Santo

Maciel,Ethel Leonor Noia; Viana,Maria Carmen; Zeitoune,Regina Célia Gomes; Ferreira,Ilce; Fregona,Geisa; Dietze,Reynaldo
Fonte: Sociedade Brasileira de Medicina Tropical - SBMT Publicador: Sociedade Brasileira de Medicina Tropical - SBMT
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2005 EN
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814.1968%
This prospective study evaluated the incidence of Mycobacterium tuberculosis infection among nursing students at the Federal University of Espírito Santo, using the purified protein derivative test. In parallel, we evaluated whether knowledge on tuberculosis transmission mechanisms had any impact on nursing students' attitudes in relation to the risk of tuberculosis infection. The incidence of tuberculin conversion was 10.5% per year (p=0.035, 95% CI = 3.63-17.43), whereas the expected conversion rate in the overall population in Brazil is 0.5% per year. These results indicate nursing students as a group at risk for TB infection. Among the risk factors studied only the use of NIOSH95 masks was associated with protection against infection (RR=0.2). Furthermore there was no statistical difference among students that PPD converted and those who remained negative regarding disease knowledge and the existence of adequate facilities for patient hospitalization. Our data reinforces the need for implementing special biosafety programs targeting this professional group.

Pulmonary Mycobacterium tuberculosis (Beijing strain) infection in a stray dog

Parsons,S D C; Gous,T A; Warren,R M; van Helden,P D
Fonte: Journal of the South African Veterinary Association Publicador: Journal of the South African Veterinary Association
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2008 EN
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819.6199%
Mycobacterium tuberculosis infection in dogs is rarely reported and has not previously been documented in South Africa. A case of a stray Maltese crossbreed dog with extensive multifocal pulmonary tuberculosis due to M. tuberculosis is described. Pulmonary granulomas in this case were poorly encapsulated and contained large numbers of acid-fast bacteria, highlighting the potential for infected companion animals to excrete the pathogen. Treatment of canine tuberculosis is generally not advised, and for this reason, euthanasia of diseased animals must be advocated in most instances. Physicians and veterinarians must be aware that companion animals with active disease caused by M. tuberculosis could act as a potential source of infection.