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Efficacy of condensed tannin presents in acacia extract on the control of Trichostrongylus colubriformis in sheep; Eficácia dos taninos condensados presentes no extrato de acácia no controle de Trichostrongylus colubriformis em ovinos

MINHO, Alessandro Pelegrine; FILIPPSEN, Laerte Francisco; AMARANTE, Alessandro Francisco Talamine do; ABDALLA, Adibe Luiz
Fonte: Universidade Federal de Santa Maria Publicador: Universidade Federal de Santa Maria
Tipo: Artigo de Revista Científica
ENG
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The aim of this study was to evaluate the potential uses of acacia extract (AE) on Trichostrongylus colubriformis control in sheep. The lambs were allocated in three groups of six animals. Two groups were experimentally infected with third-stage larvae of T. colubriformis and one was remained as non-infected control. Thirty days post-infection, the infected animals from treated group were supplemented with AE. During and after the treatment, the values of faecal eggs counts (FEC), number of third-stage larvae recovered per gram of faeces (LPG), number of FEC accumulated per animal during the treatment period, nematode egg viability, and worm burden were determined. Animals from non-infected group did not presented FEC values, during the experimental period. The average of total accumulated amount of FEC output per group, during the 10 days of the treatment was lower in AE treated lambs (P<0.05). The values of egg viability were statistically reduced (P<0.05) on days 4, 7 and 10 post treatment (p.t.). The number of LPG was lower (P<0.05), at the days 10 and 14p.t., in treated animals. AE showed potential to be used on the prophylaxis of gastrointestinal nematode infections in sheep.; O objetivo deste trabalho foi avaliar o potencial do extrato de acácia (EA) para o controle de T. colubriformis em ovinos. Os cordeiros foram alocados em três grupos de seis animais. Dois grupos foram experimentalmente infectados com larvas infectantes de T. colubriformis...

Anthelmintic effects of condensed tannins on Trichostrongylus colubriformis in experimentally infected sheep; Efeito anti-helmíntico de taninos condensados sobre Trichostrongylus colubriformis em ovinos experimentalmente infectados

MINHO, Alessandro Pelegrine; GENNARI, Solange Maria; AMARANTE, Alessandro Francisco Talamine do; ABDALLA, Adibe Luiz
Fonte: UNIV ESTADUAL LONDRINA Publicador: UNIV ESTADUAL LONDRINA
Tipo: Artigo de Revista Científica
ENG
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Recent surveys have identified anthelmintic effects from many bioactive substances particularly from condensed tannin (CT) sources. The aims of the present study were to investigate the potential anthelmintic effects of condensed tannins (CT) on Trichostrongylus colubriformis in experimentally infected sheep and the nutritional consequences on animals. Twenty helminth-free lambs were divided into five groups of four animals. Groups I to IV were artificially infected with 6,000 third stage larvae (L3) of T. colubriformis. Group I was the infected control and group V was the uninfected control. Twenty-eight days post-infection (p.i.) lambs from GII were supplemented with tanniniferous sorghum (350 g/animal/day, during seven days); GIII were drenched with Acacia mearnsii extract (15% CT) for just one day and GIV during two days (1.6 g extract/kg BW). At day 36 p.i., animals from infected group (GI to GIV) were slaughtered. Faecal egg counts (FEC) values present a reduction on GII when compared with GI at day 29 p.i. (P < 0.05) and between GIII and GI at day 35 and 36 p.i. (P < 0.05). The values of egg hatchability and number of L3 recovered from the faeces were not statistical analyzed (there was no duplicate data), however there was a considerable reduction between the values from treated and control group. The use of CT on diet did not cause significant difference on blood parameters...

Resistance of Santa Ines and crossbred ewes to naturally acquired gastrointestinal nematode infections

AMARANTE, A. F. T.; SUSIN, I.; ROCHA, R. A.; SILVA, M. B.; MENDES, C. Q.; PIRES, A. V.
Fonte: ELSEVIER SCIENCE BV Publicador: ELSEVIER SCIENCE BV
Tipo: Artigo de Revista Científica
ENG
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This trial was carried out in Piracicaba, Sao Paulo State. Brazil. to comparatively evaluate the degree of resistance to naturally acquired gastrointestinal nematode infections in sheep of the following genetic groups purebred Santa Ines (SI), SI crossbred with Dorper (DO x SI), lie de France (IF x SI), Suffolk (SU x SI), and Texel (TE < SI) Fifteen ewes from each group were raised indoors until 12 months of age. At this age, they were moved to pasture that was naturally contaminated by nematode infective larvae and were evaluated from December to May. 2007. Rainfall ranged from 267 mm in January to 37 mm in April Maximum and minimum mean temperatures ranged from 32 5 degrees C to 19 0 degrees C in March and from 25.9 degrees C to 12.8 degrees C in May. There was an increase in the mean number of eggs per gram of feces (EPG) after animals were placed on pasture with significant difference between the SI (80 EPG) and IF x SI (347 EPG) groups in January: and the DO x SI (386 EPG) and TE x SI (258 EPG) groups in May. The highest mean fecal egg count (FEC), 2073 EPG, was recorded for the TE x SI group in February. All groups showed a progressive reduction in body weight throughout the experiment of 12.0% (TE x SI) to 15.9% (SU x SI). In general. the animals with the highest FEC presented the lowest packed cell volumes (PCV): the highest correlation coefficient between FEC x PCV occurred in the SU x SI sheep in January (r = -0.70; P < 0.01). Similarly...

Sensibilidade dos nematódeos gastrintestinais de caprinos ao ivermectin na região da Grande Porto Alegre-RS; Sensitivity of gastrointestinal nematodes to ivermectin in goats in the metropolitan region of Porto Alegre, State of Rio Grande do Sul, Brazil

Mattos, Mary Jane Tweedie de; Oliveira, Carlos Marcos Barcellos de; Gouvea, Aline Silva; Andrade, Cintia Bisogno
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
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As infecções por nematódeos representam um entrave na caprinocultura, podendo levar a morte de animais. Para o seu controle é utilizado ivermectin em caprinos jovens. Este experimento foi realizado com o objetivo de verificar a eficácia deste principio ativo sobre caprinos naturalmente parasitados por nematódeos. Para isto, foram utilizados 12 animais sendo 6 medicados com ivermectin®, na dose de 200 mg/kg v.o, e 6 não medicados (controle) Para a avaliação, utilizou-se o teste de redução na contagem de ovos e redução de larvas infectantes além da carga parasitária total (adultos). Amostras fecais foram coletadas no dia da medicação (dia zero), aos 7 e 14 dias após. No 14º dia pós-medicação todos os caprinos foram sacrificados, para a coleta de helmintos e cálculo da carga parasitária total. Os resultados revelaram que o ivermectin reduziu em 93,24 % o número de ovos de Trichostrongyloidea em caprinos. Na cultura de larvas observou-se Haemonchus (41%), Ostertagia (33%) e Oesophagostomum (26%), no dia zero e somente de Haemonchus (100%) no 14ºdia após a medicação. No grupo controle, no dia da medicação foram observadas larvas de Haemonchus (71%), Ostertagia (26%) e Trichostrongylus (3%). Estes mesmos gêneros foram identificados no 14ºdia...

Recuperação de larvas de Trichostrongylus colubriformis em diferentes estratos de Brachiaria decumbens E Panicum maximum

Da Rocha, Raquel A.; Bricarello, Patrizia A.; Da Rocha, Gilberto P.; Amarante, Alessandro F. T.
Fonte: Brazilian Coll Veterinary Parasitology Publicador: Brazilian Coll Veterinary Parasitology
Tipo: Artigo de Revista Científica Formato: 77-82
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The purpose of the experiment was to evaluate infective Trichostrongylus colubriformis larvae vertical migration in two forage grass species. Experimental modules formed by eight plots, established with Brachiaria decumbens cv. Australian and Panicum maximum cv. Aruana, were used in the study, totaling four plots for each grass species. Each plot was divided into six 30 x 30 cm subplots. Larval migration was evaluated in the four seasons of the year, in different plant strata (0-7, 7-14, 14-21, 21-28 and above 28 cm). Four feces deposits were made, one in each season of the year, in the middle of 30-cm tall forage. The feces were collected from the forage ten days after each feces deposit in the experimental subplots. Grass height was measured in each of the strata immediately before the collections. The forage of the different strata was cut from an area measuring 10-cm in radius. The feces were collected manually from the subplots. There was a grass species and grass stratum interaction in the deposit made in autumn (P < 0.05). During that season, most of the larvae were recovered from the Brachiaria grass base; meanwhile, at the forage apex, the biggest average was registered in the aruana grass. Infective larvae (U) recovery was similar among the different strata during spring. In springtime...

Contaminação da pastagem com larvas infectantes de nematoides gastrintestinais após o pastejo de ovelhas resistentes ou susceptíveis à verminose

Bassetto, César Cristiano; Silva, Bruna Fernanda da; Fernandes, Simone; Amarante, Alessandro Francisco Talamini do
Fonte: Colégio Brasileiro de Parasitologia Veterinária Publicador: Colégio Brasileiro de Parasitologia Veterinária
Tipo: Artigo de Revista Científica Formato: 63-68
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O grau de contaminação da pastagem por larvas de terceiro estágio (L3) de nematoides gastrintestinais foi avaliado em piquetes pastejados por ovelhas resistentes ou susceptíveis à verminose. O experimento foi realizado de 23 de novembro de 2007 a 22 de julho de 2008. Inicialmente, realizou-se a contagem de ovos por grama de fezes (OPG) de amostras individuais de 130 ovelhas adultas da raça Bergamácia. Dessas, foram selecionadas as nove ovelhas com contagens mais elevadas de OPG (susceptíveis) e as 10 com as menores contagens (resistentes). As ovelhas resistentes apresentaram menor contagem de OPG, maiores valores de volume globular, de proteína plasmática total e de eosinófilos sanguíneos, do que as ovelhas susceptíveis. O peso também foi maior no grupo resistente. Na pastagem, foram identificadas larvas de Haemonchus spp., Trichostrongylus spp. e Oesophagostomum spp. em média, as quantidades de L3 de Haemonchus spp. e de Trichostrongylus spp., na pastagem, foram 2,19 e 2,31 vezes, respectivamente, maiores nos piquetes pastejados pelo grupo susceptível do que nos do grupo resistente. Portanto, os animais susceptíveis devem ser eliminados do rebanho a fim de reduzir a contaminação da pastagem e otimizar a profilaxia das infecções por nematoides gastrintestinais.; The degree of contamination of herbage with third stage larvae of gastrointestinal nematodes was evaluated in paddocks grazed by resistant or susceptible ewes. The trial occurred from November 23...

Recovery of Trichostrongylus colubriformis infective larvae from three grass species contaminated in the autumn

Rocha, Raquel Abdallah da; Bricarello, Patrizia Ana; Rocha, Gilberto Pedroso da; Amarante, Alessandro Francisco Talamini do
Fonte: Colégio Brasileiro de Parasitologia Veterinária Publicador: Colégio Brasileiro de Parasitologia Veterinária
Tipo: Artigo de Revista Científica Formato: 372-378
ENG
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); O experimento teve como objetivo avaliar a recuperação de larvas infectantes (L3) de Trichostrongylus colubriformis em Brachiaria decumbens cv. Australiana, Cynodon dactylon cv. Coast-cross e Panicum maximum cv. Aruana. Foram utilizados módulos experimentais constituídos por seis canteiros, perfazendo dois canteiros por espécie forrageira. A sobrevivência larval foi avaliada do outono até o inverno, sob o efeito de duas alturas de poda (5 e 30 cm). A poda foi realizada imediatamente antes da deposição das fezes contaminadas com ovos de T. colubriformis. A colheita das fezes e da forragem foi realizada uma, duas, quatro, oito, 12 e 16 semanas após a deposição das fezes nos canteiros experimentais. de modo geral, foram recuperadas larvas das forragens e das fezes até a 16ª semana. Essas larvas persistiram por mais tempo no ambiente, provavelmente em razão das temperaturas mais amenas. O número de L3 recuperadas nas pastagens não foi influenciado pela altura das plantas, exceto nos capins braquiária e aruana na quarta semana. Já em relação às concentrações de larvas (L3/kg MS) recuperadas das três forrageiras...

Recuperação de larvas infectantes de Trichostrongylus colubriformis em três espécies de gramíneas contaminadas no verão

Rocha, Raquel A. da; Rocha, Gilberto Pedroso da; Bricarello, Patrizia A.; Amarante, Alessandro Francisco Talamini do
Fonte: Colégio Brasileiro de Parasitologia Veterinária Publicador: Colégio Brasileiro de Parasitologia Veterinária
Tipo: Artigo de Revista Científica Formato: 227-234
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O experimento teve como objetivo avaliar a recuperação de larvas infectantes (L3) de Trichostrongylus colubriformis em Brachiaria decumbens cv. Australiana, Cynodon dactilon cv. Coast-cross e Panicum maximum cv. Aruana. Foram utilizados módulos experimentais constituídos por seis canteiros de 32,4 m² cada, perfazendo dois canteiros por espécie forrageira. Cada canteiro foi dividido em 36 parcelas, de 30 x 30 cm, de forma a permitir seis repetições por espécie e por altura da forragem em cada semana de colheita de material. A sobrevivência larval foi avaliada do meio do verão e até meados do outono, sob o efeito de duas alturas de poda das forragens: baixa, 5 cm e alta, 30 cm. A poda foi realizada imediatamente antes da deposição das fezes contaminadas com ovos de T. colubriformis, obtidos de ovinos, que ocorreu no dia 05/02/ 2004. A colheita das fezes e da forragem foi realizada uma, duas, quatro, oito, 12 e 16 semanas após a deposição das fezes nos canteiros experimentais. A altura da forragem foi medida em cada uma das subdivisões imediatamente antes da colheita. A forragem foi cortada rente ao solo, de uma área delimitada com o auxílio de um círculo de 10 cm de raio. As fezes foram recolhidas manualmente dos canteiros. O número de larvas infectantes recuperado foi muito pequeno em comparação com a quantidade de larvas produzidas nas culturas controle...

Immune response and performance of growing Santa Ines lambs to artificial Trichostrongylus colubriformis infections

Cardia, D. F. F.; Rocha-Oliveira, R. A.; Tsunemi, M. H.; Amarante, Alessandro Francisco Talamini do
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 248-258
ENG
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Processo FAPESP: 06/59350-7; This study was carried out to evaluate the immune response and the impact of Trichostrongylus colubriformis infections on the performance of growing Santa Ines lambs. Thirty male lambs, 3-4 months of age, were maintained in individual pens and restrictively randomised by weight into 3 treatment groups: (1) infected group, artificially infected with 2500 T. colubriformis larvae, three times a week, for 13 weeks, and fed ad libitum; (2) Pair-Fed Group, non-infected and fed with the same amount of food consumed by the infected animal of the same class on the previous day; and (3) control group, non-infected and fed ad libitum. Refused feed was weighed daily to assess the food intake of each lamb. Animals were weighed weekly and blood and fecal samples were collected. At the end of the trial, all lambs were euthanized to determine worm burden and collect intestinal tissues and mucus samples for histological and immunological analysis. The infected group presented eosinophilia, increased number of inflammatory cells in the mucosa, in addition to an increased production of specific immunoglobulins against T. colubriformis...

Sheep and cattle grazing alternately: Nematode parasitism and pasture decontamination

Rocha, R. A.; Bresciani, K. D S; Barros, T. F M; Fernandes, L. H.; Silva, M. B.; Amarante, A. F T
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 135-143
ENG
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Three grazing management systems were compared to examine pasture decontamination of gastrointestinal nematode parasites (GIN) of sheep (Ovies aries) and cattle (Bos taurus). They consisted of sheep and cattle grazing paddocks alternately for 32, 96 or 192 days over 2 years. Pastureland (8.43 ha) was subdivided into six areas of eight paddocks each to produce an eight-paddock rotational grazing system. Every paddock was grazed for 4 days and then rested for 28 days. Sixty-six Ile de France ewes and 12 steers were randomly divided into three groups (22 sheep and four cattle per group). Each grazing system included a cattle area and a sheep area. Sheep and cattle interchanged areas every 32 days in system 1 (Group 1), every 96 days in system 2 (Group 2) and every 192 days in system 3 (Group 3). Fecal examination and larvae counting on pasture were performed every 32 days. During summer, winter and spring 2005, tracer lambs free of nematode infection were introduced into each sheep group and later sacrificed for quantification and identification of GIN species. All cattle were sacrificed for the same purpose. The main parasites found in tracer lambs were Haemonchus contortus and Trichostrongylus colubriformis, and in cattle, Haemonchus similis...

Resistance against gastrointestinal nematodes in Crioulo Lageano and crossbred Angus cattle in southern Brazil

Cardoso, Cristina P.; Silva, Bruna F.; Trinca, Luzia A.; Amarante, Alessandro F.T.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 183-191
ENG
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Gastrointestinal nematode (GIN) infection is a major cause of production losses in cattle. This study was carried out to evaluate the natural resistance against nematode infection in Crioulo Lageano and crossbred Angus male calves. Crioulo Lageano is a local cattle breed in the state of Santa Catarina, in southern Brazil. Ten weaned calves of each breed were grazed together on pasture and naturally infected with nematodes between July 2009 and December 2010. Once every 28 days, we collected fecal and blood samples for parasitological and immunological tests, as well as recording body weights. After 19 samplings, all animals were slaughtered for quantification and identification of GINs. We found that the animals had been infected with the following nematode species, in decreasing order by the mean number of specimens: Trichostrongylus axei, Cooperia punctata, Ostertagia ostertagi, Haemonchus placei, Oesophagostomum radiatum, and Trichuris spp. There were no significant differences between the Crioulo Lageano and crossbred Angus groups in terms of worm burden or nematode fecal egg count, nor in terms of the mean levels of immunoglobulin (G and A) against C. punctata and H. placei antigens, except in IgA mean level in abomasal mucus against H. placei adult worms that was significantly higher in crossbred Angus cattle (p<0.05). At the end of the study...

Trichostrongylus and Haemonchus anthelmintic resistance in naturally infected sheep from southern Brazil

KLAUCK,VANDERLEI; PAZINATO,RAFAEL; LOPES,LEANDRO S.; CUCCO,DIEGO C.; LIMA,HORACIO L. DE; VOLPATO,ANDREIA; RADAVELLI,WILLIAN M.; STEFANI,LENITA C.M.; SILVA,ALEKSANDRO S. DA
Fonte: Academia Brasileira de Ciências Publicador: Academia Brasileira de Ciências
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 EN
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The anthelmintic resistance in small ruminants is a common problem and concern worldwide. The aim of this study was to verify anthelmintic treatment efficacy in naturally infected sheep. This study was conducted on nine herds that used the same anthelmintic management for over a year. In each farm, the animals were divided into two groups: untreated control group (n = 5) and treated (n = 10) according to the number of eggs per gram of feces (EPG). The treatment effect was checked based on EPG results and larval culture performed before treatment and 10 days after treatment. Significant differences were not observed (P > 0.05) on EPG results between untreated and treated groups. The coproculture showed that the animals were infected primarily by Haemonchus spp., Trichostrongylus spp., Teladorsagia spp., Cooperia spp. and Oesophagostomum spp. In all farms, anthelmintic resistance by genera Haemonchus and Trichostrongylus was found, but this resistance varied greatly between farms. Haemonchus spp. showed resistance to closantel, levamisole, and albendazole. Trichostrongylus spp. was shown to be resistant to closantel, levamisole, and albendazole. The drugs tested showed to be efficient against the genera Teladorsagia, Cooperia, and Oesophagostomum. Based on these results...

Recovery of Trichostrongylus colubriformis infective larvae from three grass species contaminated in the autumn

Rocha,Raquel Abdallah da; Bricarello,Patrizia Ana; Rocha,Gilberto Pedroso da; Amarante,Alessandro Francisco Talamini do
Fonte: Colégio Brasileiro de Parasitologia Veterinária Publicador: Colégio Brasileiro de Parasitologia Veterinária
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2012 EN
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This experiment aimed to assess the recovery of infective larvae (L3) of Trichostrongylus colubriformis from Brachiaria decumbens cv. Australiana, Cynodon dactylon cv. Coast-cross and Panicum maximum cv. Aruana. The experimental module comprised six plots, with two plots per herbage species. Larval survival was assessed from autumn to winter, under the effect of two herbage-paring heights (5 and 30 cm). TThe paring was carried out immediately before contamination with faces containing T. colubriformis eggs. The feces and herbage were collected at one, two, four, eight, 12 and 16 weeks after feces had been deposited in the experimental plots. In general, larvae were recovered from both herbage and feces until the 16th week. The longer persistence of these larvae in the environment was probably due to warmer temperatures. The number of L3 recovered from the pasture was not influenced by the height of plants, except for Brachiaria and Aruana herbage in the fourth week. Regarding the concentrations of larvae per kg of dry matter (L3/kg DM), recovery was higher from low pasture in all three herbage species. During the autumn, the development and survival of the T. colubriformis free-living stages were not affected by the different herbage species.

Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence

Yong, Tai-Soon; Lee, Jong-Ho; Sim, Seobo; Lee, Jongweon; Min, Duk-Young; Chai, Jong-Yil; Eom, Keeseon S.; Sohn, Woon-Mok; Lee, Soon-Hyung; Rim, Han-Jong
Fonte: The Korean Society for Parasitology Publicador: The Korean Society for Parasitology
Tipo: Artigo de Revista Científica
EN
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Trichostrongylus eggs observed in cellophane-thick smears are difficult, in practice, to distinguish from hookworm eggs. In order to overcome these limitations, a molecular approach was conducted. A Trichostrongylus colubriformis adult worm was obtained from a human in Laos, which was identified morphologically. ITS-1 sequence of this worm was determined, and found to be most similar with that of T. colubriformis among the Trichostrongylus spp. reported so far. Then, this sequence was compared with those of human hookworm species, Ancylostoma duodenale and Necator americanus, and species-specific oligonucleotide primers were designed. Polymerase chain reaction (PCR) using these primers evidenced specifically amplified PCR products of Trichostrongylus sp., A. duodenale and N. americanus from the eggs of each (520 bp, 690 bp, and 870 bp, respectively). A species-specific PCR technique can be developed in order to study the epidemiology of Trichostrongylus spp. and hookworms in endemic areas.

Human Trichostrongylus colubriformis Infection in a Rural Village in Laos

Sato, Megumi; Yoonuan, Tippayarat; Sanguankiat, Surapol; Nuamtanong, Supaporn; Pongvongsa, Tiengkham; Phimmayoi, Inthava; Phanhanan, Vilayphone; Boupha, Boungnong; Moji, Kazuhiko; Waikagul, Jitra
Fonte: The American Society of Tropical Medicine and Hygiene Publicador: The American Society of Tropical Medicine and Hygiene
Tipo: Artigo de Revista Científica
Publicado em 05/01/2011 EN
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In Lahanam Village, Savannakhet Province, Laos, 125 of 253 villagers (49.4%) were found by fecal examination to harbor hookworm eggs. The eggs were heterogeneous in morphology and size, suggesting infections of mixed nematode species. To confirm the hookworm egg species, on a voluntary basis, 46 hookworm egg–positive participants were treated with albendazole, and post-treatment adult worms were collected from purged fecal samples. The common human hookworm was found in only 3 participants; 1 case of Necator americanus, and 2 cases of Ancylostoma duodenale. In contrast, adult Trichostrongylus worms were expelled from most participants (43 of 46, 93.5%). The Trichostrongylus species were confirmed by morphology and internal transcribed spacer 2 sequences; all worms were of the same species (T. colubriformis). In addition, some Trichostrongylus worms were obtained from a goat in the same village and identified as T. colubriformis. The results suggested that T. colubriformis was the main zoonotic species causing hookworm infections in the village.

TH1 and TH2 cytokines mRNA levels in Brazilian Somalis crossbreed sheep resistant and suceptible to Trichostrongylus colubriformis infection.

ZAROS, L. G.; COUTINHO, L. L.; NAVARRO, A. M. C.; NEVES, M. R. N.; BENVENUTI, C. L.; SIDER, L. H.; VIEIRA, L. da S.
Fonte: In: CONFERENCE FOR THE INTERNATIONAL SOCIETY FOR ANIMAL GENETICS, 32., 2010. Edinburgo (UK). Proceedings... Edinburgo: ISAG, 2010. p. 132. Abstract P5052. Publicador: In: CONFERENCE FOR THE INTERNATIONAL SOCIETY FOR ANIMAL GENETICS, 32., 2010. Edinburgo (UK). Proceedings... Edinburgo: ISAG, 2010. p. 132. Abstract P5052.
Tipo: Resumo em anais de congresso (ALICE)
EN
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Cytokines are proteins that play a central role in immune mechanisms involved in defense against gastrointestinal nematodes infections. The present study used the real-time PCR methodology to quantify Brazilian Somalis crossbreed sheep cytokines (IL-4, IL-13, TNF-? and IFN-?) in two groups: one resistant and other susceptible to Trichostrongylus colubriformis infection. From a Somalis sheep herd, 75 young animals were kept together on pasture without anthelmintic treatment for 4 months. The eight most resistant and the eight most susceptible animals were chosen based on the mean of fecal egg counts and slaughtered for recover the parasites and small intestine tissue samples collection. RT-PCR was performed using the LightCycler PCR and SYBR Green I dye. SDHA (succinate dehydrogenase complex subunit A) was used for normalization and the relative quantifi cation of genes was calculated by REST software. Resistant animals presented lower EPG counts than susceptible animals (1312,5 and 5081,6, respectively; P<0.0001) and 3 fold less specimens of Trichostrongylus colubriformis (P<0.05). Only IL-13 was up-regulated in resistant animals (P<0.02) and the other three genes analyzed, IL-4, TNF-? and IFN-? were down-regulated in this group, although not signifi cantly (P>0.05). IL-13 is a cytokine that stimulates the TH2 response...

Parâmetros parasitológicos e desempenho produtivo de ovinos naturalmente infectados com nematoides gastrintestinais mantidos em diferentes pastagens tropicais; Parasitological parameters and productive performance of sheep naturally infected with gastrointestinal nematodes in different tropical pastures

Andrade Junior, Alberto Luiz Freire de
Fonte: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Produção Animal; Sistemas de Produção Sustentáveis no Semi-árido; Caracterização, conservação e melhoramento genético Publicador: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Produção Animal; Sistemas de Produção Sustentáveis no Semi-árido; Caracterização, conservação e melhoramento genético
Tipo: Dissertação Formato: application/pdf
POR
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284.9823%
The control of gastrointestinal nematodes of small ruminants is usually done with anthelmintics. However, due to the emergence of ever-increasing parasite resistance to these drugs, looking up an alternative control parasites. One of this is sought in pasture management, as these are the sources of animals` infection by L3 infective larvae of helminths. The aim of this study was to evaluate the performance of sheep to natural infections by gastrointestinal nematodes grazing Panicum maximum cv. Massai and cv. Aruana, and Brachiaria brizantha cv. Piatã and cv. Marandu. The work was conducted from May to August-2011 with 48 male sheeps SRD versus Santa Inês breed. The animals were naturally infected with gastrointestinal nematodes, and maintained in four different cultivars of tropical forage grasses, naturally contaminated with eggs and larvae of gastrointestinal nematodes. Each week the animals were phenotypically characterized by parasitological examinations (eggs per gram of feces - EPG, and feces culture), hematological (packed cell volume PCV, and blood eosinophil count) method to evaluate the Famacha© colorof ocular mucosa, and the measures of body condition score and weight. In pastures was made the recovery of infective larvae in order to determine the quantity of L3 present in the pasture. The experimental design was a randomized completed block with two replications and before the entry of animals in the paddocks...

Gastrointestinal nematode infections in sheep raised in Botucatu, state of Sao Paulo, Brazil

Wilmsen, Maurício Orlando; Silva, Bruna Fernanda; Bassetto, César Cristiano; Amarante, Alessandro Francisco Talamini do
Fonte: Brazilian Coll Veterinary Parasitology Publicador: Brazilian Coll Veterinary Parasitology
Tipo: Artigo de Revista Científica Formato: 348-354
ENG
Relevância na Pesquisa
278.44521%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Processo FAPESP: 2008/53494-2; Processo FAPESP: 2007/58244-1; Processo FAPESP: 2009/03504-4; Gastrointestinal nematode infections were evaluated in sheep raised in Botucatu, state of Sao Paulo, Brazil between April 2008 and March 2011. Every month, two tracer lambs grazing with a flock of sheep were exposed to natural infection with gastrointestinal nematodes for 28 consecutive days. At the end of this period, the lambs were sacrificed for worm counts. Haemonchus contortus presented 100% of prevalence. The seasons exerted no significant influence on the mean intensity of H. contortus, which ranged from 315 worms in November 2010 to 2,5205 worms in January 2011. The prevalence of Trichostrongylus colubriformis was also 100%, with the lowest mean intensity (15 worms) recorded in February 2011 and the highest (9,760 worms) in October 2009. In the case of T. colubriformis, a significant correlation coefficient was found between worm counts vs. rainfall (r = -0.32; P < 0.05). Three other nematodes species were found in tracer lambs, albeit in small numbers. Their prevalence and mean intensity (in parenthesis) were as follows: Oesophagostomum columbianum 28% (25.2)...

Ovine nematodes in wild lagomorphs in Australia and first record of Trichostrongylus rugatus in free living lagomorphs

Tai, M.; O'Handley, R.; Hemmatzadeh, F.; Jenkins, D.; Stott, P.
Fonte: Elsevier Science BV Publicador: Elsevier Science BV
Tipo: Artigo de Revista Científica
Publicado em //2013 EN
Relevância na Pesquisa
382.24094%
Gastrointestinal parasites are a major problem for sheep graziers and anthelmintic resistance has been reported worldwide. Given that in experimental circumstances, sheep helminths are able to develop in rabbits and hares, and that hares are very mobile and sympatric with sheep in many regions of Australia, free-living lagomorphs were investigated regarding carriage of ovine nematode parasites under field conditions. We performed specific worm counts in the gastrointestinal tracts of 88 rabbits and 110 hares. We revealed that ruminant worms are common in hares (prevalence 79%) and that they are occasionally found in rabbits (9%). Statistical analyses showed that the ruminant worm Trichostrongylus colubriformis occurred frequently in hares whilst rabbits were commonly infected with lagomorph-specific Trichostrongylus retortaeformis. Detection of the ovine worm Trichostrongylus rugatus is reported for the first time in wild lagomorphs. The potential for cross-transmission between hares and sheep in the natural environment is much more prevalent than previously believed.; Marina H.H. Tai, Ryan O’Handley, Farhid Hemmatzadeh, David J. Jenkins, Philip Stott

Relationship of intestinal histology with the resistance to Trichostrongylus colubriformis infection in three breeds of sheep

Amarante,Alessandro F.T.; Rocha,Raquel A.; Bricarello,Patrizia A.
Fonte: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Publicador: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2007 EN
Relevância na Pesquisa
372.842%
The study was carried out to evaluate the relationship of inflammatory intestinal cells with the resistance to Trichostrongylus colubriformis infections in three breeds of sheep (Santa Ines, Suffolk and Ile de France), naturally infected. Mast cells, eosinophils, and globule leucocytes were enumerated in intestinal mucosa. Histamine concentration was estimated in intestinal tissue samples and the length of male and female specimens were determined. The three breeds of sheep showed similar cellular response in the small intestine mucosa (P>0.05). There was extensive variation among sheep in the parasitological and inflammatory cell variables, even in lambs of the same breed. In general, animals presenting less inflammatory cells had a larger worm burden, higher fecal egg counts, and larger T. colubriformis worms. The inflammatory cells possibly impaired the parasite's establishment, development, and survival.